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Identification of gene functions associated to active and dormant buds in Arabidopsis.

ABSTRACT: In Arabidopsis, axillary buds may become dormant in response to a far-red rich light or to increased apical dominance. BRANCHED1 (BRC1) is required for this response. Transcriptional profiling studies of wild-type and brc1 mutant buds allowed the identification of sets of BRC1-dependent genes including a group of ABA-related genes upregulated in dormant buds. By using BRC1 inducible lines we demonstrate that 2 of these ABA response factors, ABF3 and ABI5, are positively regulated in axillary buds by BRC1 after induction. To get further insight into the genetic control of the growth-to-dormancy transition in buds we have also compared this transcriptomic data with 2 additional "active vs dormant bud" transcriptomic data sets and found "core" co-regulated gene networks tightly associated to each condition.

SUBMITTER: Gonzalez-Grandio E

PROVIDER: S-EPMC4092316 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Identification of gene functions associated to active and dormant buds in Arabidopsis.

González-Grandío Eduardo E Cubas Pilar P

Plant signaling & behavior 20140211 2

In Arabidopsis, axillary buds may become dormant in response to a far-red rich light or to increased apical dominance. BRANCHED1 (BRC1) is required for this response. Transcriptional profiling studies of wild-type and brc1 mutant buds allowed the identification of sets of BRC1-dependent genes including a group of ABA-related genes upregulated in dormant buds. By using BRC1 inducible lines we demonstrate that 2 of these ABA response factors, ABF3 and ABI5, are positively regulated in axillary bud ...[more]

PMID: 24518068

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Project description:BackgroundMycobacterium tuberculosis, the causative agent of tuberculosis (TB), infects approximately 2 billion people worldwide and is the leading cause of mortality due to infectious disease. Current TB therapy involves a regimen of four antibiotics taken over a six month period. Patient compliance, cost of drugs and increasing incidence of drug resistant M. tuberculosis strains have added urgency to the development of novel TB therapies. Eradication of TB is affected by the ability of the bacterium to survive up to decades in a dormant state primarily in hypoxic granulomas in the lung and to cause recurrent infections.MethodsThe availability of M. tuberculosis genome-wide DNA microarrays has lead to the publication of several gene expression studies under simulated dormancy conditions. However, no single model best replicates the conditions of human pathogenicity. In order to identify novel TB drug targets, we performed a meta-analysis of multiple published datasets from gene expression DNA microarray experiments that modeled infection leading to and including the dormant state, along with data from genome-wide insertional mutagenesis that examined gene essentiality.ResultsBased on the analysis of these data sets following normalization, several genome wide trends were identified and used to guide the selection of targets for therapeutic development. The trends included the significant up-regulation of genes controlled by devR, down-regulation of protein and ATP synthesis, and the adaptation of two-carbon metabolism to the hypoxic and nutrient limited environment of the granuloma. Promising targets for drug discovery were several regulatory elements (devR/devS, relA, mprAB), enzymes involved in redox balance and respiration, sulfur transport and fixation, pantothenate, isoprene, and NAD biosynthesis. The advantages and liabilities of each target are discussed in the context of enzymology, bacterial pathways, target tractability, and drug development.ConclusionBased on our bioinformatics analysis and additional discussion of in-depth biological rationale, several novel anti-TB targets have been proposed as potential opportunities to improve present therapeutic treatments for this disease.

Project description:Glacier and ice sheet surfaces host diverse communities of microorganisms whose activity (or inactivity) influences biogeochemical cycles and ice melting. Supraglacial microbes endure various environmental extremes including resource scarcity, frequent temperature fluctuations above and below the freezing point of water, and high UV irradiance during summer followed by months of total darkness during winter. One strategy that enables microbial life to persist through environmental extremes is dormancy, which despite being prevalent among microbial communities in natural settings, has not been directly measured and quantified in glacier surface ecosystems. Here, we use a combination of metabarcoding and metatranscriptomic analyses, as well as cell-specific activity (BONCAT) incubations to assess the diversity and activity of microbial communities from glacial surfaces in Iceland and Greenland. We also present a new ecological model for glacier microorganisms and simulate physiological state-changes in the glacial microbial community under idealized (i) freezing, (ii) thawing, and (iii) freeze-thaw conditions. We show that a high proportion (>50%) of bacterial cells are translationally active in-situ on snow and ice surfaces, with Actinomycetota, Pseudomonadota, and Planctomycetota dominating the total and active community compositions, and that glacier microorganisms, even when frozen, could resume translational activity within 24 h after thawing. Our data suggest that glacial microorganisms respond rapidly to dynamic and changing conditions typical of their natural environment. We deduce that the biology and biogeochemistry of glacier surfaces are shaped by processes occurring over short (i.e., daily) timescales, and thus are susceptible to change following the expected alterations to the melt-regime of glaciers driven by climate change. A better understanding of the activity of microorganisms on glacier surfaces is critical in addressing the growing concern of climate change in Polar regions, as well as for their use as analogues to life in potentially habitable icy worlds.

Dataset Information

Identification of gene functions associated to active and dormant buds in Arabidopsis.

Publications

Identification of gene functions associated to active and dormant buds in Arabidopsis.

Similar Datasets

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