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The Influence of Dietary Calcium and Phosphorus Imbalance on Intestinal NaPi-IIb and Calbindin mRNA Expression and Tibia Parameters of Broilers.

ABSTRACT: A 2×2 factorial experiment was conducted to study the effect of dietary calcium and non-phytate phosphorus (nPP) imbalance on calbindin and NaPi-IIb mRNA levels in the small intestine and tibia parameters of broiler chicks. One hundred and forty four 1-d-old Arbor Acres male broiler chicks were divided into four treatments consisted of six replicates with six chicks each. The two dietary calcium levels were 1.10% and 0.60%, and two dietary nPP levels were 0.50% and 0.27%. Results showed that a high Ca/nPP ratio diet (4.07:1) significantly depressed feed intake and weight gain of broilers (p<0.05), but a lower Ca:nPP ratio (1.2:1) had no influence (p>0.05). Low-Ca with low-P diet resulted in low tibia minerals and tibia breaking strength of broilers, and all the tibia parameters were further decreased when the dietary ratio of Ca to P was relative higher. Low dietary Ca or P up-regulated the calbindin and NaPi-IIb mRNA expression levels. Low Ca with normal P diet up-regulated duodenal calbindin mRNA expression level to the greatest extent. Low P with a normal Ca diet significantly enhanced NaPi-IIb mRNA expression level to the highest extent. These results suggest that the calbindin and NaPi-IIb mRNA expression were enhanced by the imbalance between dietary Ca and nPP, and their expression were not only influenced by Ca or nPP level, but also the ratio of Ca:nPP.

SUBMITTER: Li J

PROVIDER: S-EPMC4092907 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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The Influence of Dietary Calcium and Phosphorus Imbalance on Intestinal NaPi-IIb and Calbindin mRNA Expression and Tibia Parameters of Broilers.

Li Jianhui J Yuan Jianmin J Guo Yuming Y Sun Qiujuan Q Hu Xiaofei X

Asian-Australasian journal of animal sciences 20120401 4

A 2×2 factorial experiment was conducted to study the effect of dietary calcium and non-phytate phosphorus (nPP) imbalance on calbindin and NaPi-IIb mRNA levels in the small intestine and tibia parameters of broiler chicks. One hundred and forty four 1-d-old Arbor Acres male broiler chicks were divided into four treatments consisted of six replicates with six chicks each. The two dietary calcium levels were 1.10% and 0.60%, and two dietary nPP levels were 0.50% and 0.27%. Results showed that a h ...[more]

PMID: 25049596

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Project description:Adequate provision of calcium (Ca) and phosphorus (P) is essential for bone formation and high growth performance in pigs. Nevertheless, reliable serum biomarkers for pig's Ca and P intake are still missing. Here, we used phytase supplementation to alter the dietary available P (aP) level in order to investigate the effect of differences in dietary aP levels on serum parameters related to the Ca and P homeostasis in pigs. Moreover, we assessed whether serum parameters can be used to predict the Ca, total P (tP), and aP intake in barrows and gilts throughout the fattening period. In total, 216 pigs (115 gilts and 101 barrows) were randomly allotted to one of the two diets in three replicate batches, each lasting 56 d (n = 108/diet). Pigs had free access to the diets without (Con) or with phytase (Phy; 650 phytase units/kg) via a transponder-based feeding system. Blood samples were collected on days 2, 23, and 52, and serum parameters were correlated with the daily Ca, tP, and aP intake. The intake of tP, aP, and Ca was overall 14.2%, 13.8%, and 14.2% higher in barrows compared with gilts, respectively (P < 0.001). Concurrently, phytase decreased the intake of tP and Ca by 8.4% and 6.7%, respectively, whereas it raised the intake of aP by 16.3% compared with the Con diet (P < 0.001). Serum levels of fibroblast growth factor 23, alkaline phosphatase (ALP), vitamin D (VitD), and osteocalcin (OCN) decreased with age (P < 0.05). The higher aP intake of pigs fed the Phy diet increased serum P on days 2 and 23 but decreased it on day 52 compared with the Con diet (P = 0.004). Pigs fed the Phy diet had higher serum ALP compared with pigs fed the Con diet on days 23 and 52 (P < 0.05). Correlation analysis between serum parameters and Ca, tP, and aP intake showed age- and sex-related associations. With 12 wk of age, serum P in both sexes, serum VitD in barrows, and serum OCN and ALP in gilts correlated with aP intake (|r| > 0.38), whereas serum OCN correlated with Ca in both sexes' intake (r > 0.50). At 20 wk, serum Ca and ALP in gilts correlated with aP intake, whereas serum P, Ca, and VitD correlated with Ca intake in both sexes (|r| > 0.39). In conclusion, the present results showed that the daily Ca and aP intake could be most reliably estimated from serum parameters for an approximate age of 12 and 20 wk. Serum P and the Ca:P ratio at 12 wk of age and serum VitD at 20 wk of age may be used to predict pig's daily aP intake in both sexes.

Project description:During the incubation period, the Ca-to-P weight (mg/mg) ratio in the yolk increases from 0.26 on day 0 to 0.92 on day 17.5 and to 2.9 at hatch. Moreover, the absolute Ca content in the yolk increases by 41%, whereas P content decreases by 87%, from day 0 to the day of hatching. Thus, at hatch and during the first days after hatching, there are high reserves of Ca relative to P in the residual egg yolk, risking hypercalcemia and hypophosphatemia. A growth performance study was conducted to explore the effects of reducing dietary Ca content in the prestarter phase (0-4 D) on BW and bone mineral deposition during the first days after hatch and at market weight (day 37). Four prestarter (0-4 D) diets were formulated to have 0.4, 0.6, 0.8, and 1.0% Ca content. After the prestarter phase, all birds were fed with the same commercial diets based on a 3-phase feeding program (starter, grower, and finisher). Growth performance (BW, ADG, ADFI, and feed conversion ratio [FCR]) was monitored throughout the study, and blood and tibia bone samples were collected on specific days. On day 4, BW and ADG decreased with dietary Ca contents higher than 0.6% (P < 0.05), but there were no differences in BW on day 14 onward (P > 0.10). For the overall study (0-37 D), there were no differences in ADG and ADFI, but the FCR decreased with lower Ca contents (P < 0.05). On day 4, there were no differences in blood plasma Ca concentration, but P concentration increased in the group treated with diet containing 0.4% Ca compared with the groups treated with diets containing 0.6 and 0.8% Ca (P < 0.05). Tibia ash content decreased in the group treated with diet containing 0.4% Ca (P < 0.05) compared with all other treatments at the end of the prestarter phase. Tibia ash content and tibia breaking strength, on day 37, were not different among the treatments (P > 0.10). In conclusion, during the prestarter phase, BW increased with dietary Ca contents lower than 0.6%, most likely improving Ca-P balance; bone mineral deposition was reduced in this period. On feeding with a diet containing higher Ca content, bone mineral content was rapidly recovered.

Dataset Information

The Influence of Dietary Calcium and Phosphorus Imbalance on Intestinal NaPi-IIb and Calbindin mRNA Expression and Tibia Parameters of Broilers.

Publications

The Influence of Dietary Calcium and Phosphorus Imbalance on Intestinal NaPi-IIb and Calbindin mRNA Expression and Tibia Parameters of Broilers.

Similar Datasets

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