Dataset Information

Phylogeography and Population Genetic Structure of Amur Grayling Thymallus grubii in the Amur Basin.

ABSTRACT: Amur grayling, Thymallus grubii, is an important economic cold freshwater fish originally found in the Amur basin. Currently, suffering from loss of habitat and shrinking population size, T. grubii is restricted to the mountain river branches of the Amur basin. In order to assess the genetic diversity, population genetic structure and infer the evolutionary history within the species, we analysised the whole mitochondrial DNA control region (CR) of 95 individuals from 10 rivers in China, as well as 12 individuals from Ingoda/Onon and Bureya River throughout its distribution area. A total of 64 variable sites were observed and 45 haplotypes were identified excluding sites with gaps/missing data. Phylogenetic analysis was able to confidently predict two subclade topologies well supported by maximum-parsimony and Bayesian methods. However, basal branching patterns cannot be unambiguously estimated. Haplotypes from the mitochondrial clades displayed local homogeneity, implying a strong population structure within T. grubii. Analysis of molecular variance detected significant differences among the different geographical rivers, suggesting that T. grubii in each river should be managed and conserved separately.

SUBMITTER: Ma B

PROVIDER: S-EPMC4092984 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Phylogeography and Population Genetic Structure of Amur Grayling Thymallus grubii in the Amur Basin.

Ma Bo B Lui Tingting T Zhang Ying Y Chen Jinping J

Asian-Australasian journal of animal sciences 20120701 7

Amur grayling, Thymallus grubii, is an important economic cold freshwater fish originally found in the Amur basin. Currently, suffering from loss of habitat and shrinking population size, T. grubii is restricted to the mountain river branches of the Amur basin. In order to assess the genetic diversity, population genetic structure and infer the evolutionary history within the species, we analysised the whole mitochondrial DNA control region (CR) of 95 individuals from 10 rivers in China, as well ...[more]

PMID: 25049647

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Project description:This trial was conducted to assess the effects of different levels of dietary fish oil on growth performance, hepatic health, nonspecific immune responses, and intestinal microbial community of Amur grayling (Thymallus grubii). Five isonitrogenous diets containing 60 (6FO), 90 (9FO), 120 (12FO), 150 (15FO), and 180 g/kg (18FO) fish oil were fed to triplicate groups of 60 fish per tank for 8 weeks, respectively. The results revealed that specific growth rate (SGR) and weight gain (WG) of fish in the 15FO group were significantly greater than those in the 6FO group (p < 0.05). Somatic indices and whole-body lipid levels were positively correlated with increases in dietary fish oil levels. Trypsin and lipase activities in 15FO and 18FO groups were significantly higher than those in the 6FO and 9FO groups (p < 0.05). The activities of intestinal catalase (CAT) and liver superoxide dismutase (SOD), CAT, lysozyme (LZM), alkaline phosphatase (AKP), and acid phosphatase (ACP) improved significantly as the dietary lipid content increased to 185.3 g/kg and decreased thereafter (p < 0.05). The lipid metabolism-related genes peroxisome proliferator-activated receptor gamma (PPARγ) and carnitine palmitoyltransferase 1A (CPT1A) were significantly downregulated and upregulated (p < 0.05), respectively, in the 15FO group. Immune-related genes in the liver and intestine, such as interleukin (IL-8), were significantly upregulated in the 15FO group (p < 0.05). The liver sections from 18FO group presented more numerous and larger lipid vacuoles. Both low- (6FO) and high-lipid (18FO) diets reduced the relative abundance of intestinal Lactococcus. The relative abundances of intestinal Staphylococcus and Bacillus (mainly Bacillus anthracis) increased in the low-lipid diet group and that of Pedobacter increased in the high-lipid diet group. Second-order polynomial analysis of WG and the feed conversion ratio (FCR) for varying levels of dietary lipid revealed that a range of 194.76-198.90 g/kg dietary lipid was optimal for the growth and health of Amur grayling.

Project description:BackgroundThe structure and diversity of grayling (Thymallus thymallus) populations have been well studied in most of its native habitat; however the southernmost populations of the Balkan Peninsula remain largely unexplored. The purpose of this study was to assess the genetic diversity of Serbian grayling populations, detect the impact of stocking and provide guidelines for conservation and management.MethodsEighty grayling individuals were collected from four rivers (Ibar, Lim, Drina and Rzav). The mitochondrial DNA control region (CR; 595 bp of the 3'end and 74 bp of flanking tRNA) and the ATP6 gene (630 bp fragment) were sequenced for 20 individuals (five from each locality). In addition, all individuals were genotyped with 12 microsatellite loci. The diversity and structure of the populations as well as the recent and ancient population declines were studied using specialized software.ResultsWe detected three new haplotypes in the mtDNA CR and four haplotypes in the ATP6 gene of which three had not been described before. Previously, one CR haplotype and two ATP6 gene haplotypes had been identified as allochthonous, originating from Slovenia. Reconstruction of phylogenetic relations placed the remaining two CR haplotypes from the River Danube drainage of Serbia into a new clade, which is related to the previously described sister Slovenian clade. These two clades form a new Balkan clade. Microsatellite marker analysis showed that all four populations are genetically distinct from each other without any sign of intra-population structure, although stocking of the most diverse population (Drina River) was confirmed by mtDNA analysis. Recent and historical population declines of Serbian grayling do not differ from those of other European populations.ConclusionsOur study shows that (1) the Ibar, Lim and Drina Rivers grayling populations are genetically distinct from populations outside of Serbia and thus should be managed as native populations in spite of some introgression in the Drina River population and (2) the Rzav River population is not appropriate for further stocking activities since it originates from stocked Slovenian grayling. However, the Rzav River population does not represent an immediate danger to other populations because it is physically isolated from these.

Dataset Information

Phylogeography and Population Genetic Structure of Amur Grayling Thymallus grubii in the Amur Basin.

Publications

Phylogeography and Population Genetic Structure of Amur Grayling Thymallus grubii in the Amur Basin.

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