Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastoris Expression System.
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ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC4093532 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Publications
Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastoris Expression System.
Li Jianbo J Jiang Mingfeng M Wang Yong Y
Asian-Australasian journal of animal sciences 20140401 4
A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific activity greater than 500 mg/L and 4,000 U/mg was obtained. Results at optimal pH and temperature showed recombinant lysozyme has higher lytic activity at pH 6.5 and 45°C. This study demonstrates the successful expression of recombinant lysozyme using the eukaryotic host organism P. pastoris paving ...[more]
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