Unknown

Dataset Information

0

Cloning and Sequence Analysis of Wild Argali ISG15 cDNA.

ABSTRACT: The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1), and Sus scrofa (accession no. EU647216.1), respectively. The entire coding sequence was inserted into the pET-28a vector and expressed in E. coli. The recombinant protein corresponded to the expected molecular mass of 25 kDa as judged by SDS-PAGE, and it was detected in the bacterial inclusion bodies. The expressed protein could be purified by Ni(2+) chelate affinity chromatography and the results from the lymphocyte proliferation test showed that the product could stimulate lymphocyte proliferation very well (p<0.05), which further confirmed its biological activity.

SUBMITTER: Sun Y 

PROVIDER: S-EPMC4093538 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Cloning and Sequence Analysis of Wild Argali ISG15 cDNA.

Sun Yanming Y   Chen Kaili K   Shen Wen W   Cui Rupeng R   Lu Haifu H  

Asian-Australasian journal of animal sciences 20140401 4

The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1)  ...[more]

Similar Datasets

Unknown Molecular cloning and sequence analysis of hamster CENP-A cDNA.
| S-EPMC113255 | biostudies-literature
Unknown cDNA cloning and sequence analysis of human pancreatic procarboxypeptidase A1.
| S-EPMC1133158 | biostudies-other
Unknown Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information.
| S-EPMC1217213 | biostudies-literature
Unknown Cloning and further sequence analysis of the ORF3 gene of wild- and attenuated-type porcine epidemic diarrhea viruses.
| S-EPMC7088598 | biostudies-literature
Unknown Cloning and sequence analysis of the cDNA for arachidonate 12-lipoxygenase of porcine leukocytes.
| S-EPMC53642 | biostudies-other
Unknown cDNA cloning, sequence analysis, and chromosomal localization of the gene for human carnitine palmitoyltransferase.
| S-EPMC50872 | biostudies-other
Unknown Cloning and sequence analysis of a Bothrops jararaca cDNA encoding a precursor of seven bradykinin-potentiating peptides and a C-type natriuretic peptide.
| S-EPMC19766 | biostudies-literature
Unknown Serine protease variants encoded by Echis ocellatus venom gland cDNA: cloning and sequencing analysis.
| S-EPMC2949595 | biostudies-literature
Unknown Molecular cloning and sequence analysis of the cDNA for small proteoglycan II of bovine bone.
| S-EPMC1148620 | biostudies-other
Unknown Cloning and sequence analysis of a cDNA clone coding for the mouse GM2 activator protein.
| S-EPMC1134589 | biostudies-other