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Expression of P450 and nuclear receptors in normal and end-stage Chinese livers.

ABSTRACT: To investigate the expression of P450 enzyme genes by using end-stage liver disease samples and trimmed normal Chinese donor livers.The end-stage liver disease samples [n = 93, including hepatocellular carcinoma (HCC), peri-HCC tissue, hepatitis B virus cirrhosis, alcoholic cirrhosis, and severe cirrhosis] and trimmed normal Chinese donor livers (n = 35) from The Institute of Organ Transplantation in Beijing, China. Total RNA was extracted, purified, and subjected to real-time RT-PCR analysis.For cytochrome P450 enzymes 1 (CYP1) family, the expression of CYP1A2 was decreased 90% in HCC, 80% in alcoholic cirrhosis, and 65% in severe cirrhosis. For CYP2 family, the expression of CAR was decreased 50% in HCC, but increased 50% in peri-HCC tissues. Similar decreases (about 50%) of CYP2B6, CYP2C9, CYP2C19, CYP2D6 and CYP2E1 were observed in HCC, as compared to peri-HCC tissues and normal livers. CYP2C19 were decreased in all end-stage liver diseases and CYP2E1 also decreased in alcoholic cirrhosis and severe cirrhosis. For CYP3 family, the expression of PXR was decreased 60% in HCC, together with decreases in CYP3A4, CYP3A5, and CYP3A7. In contrast, the expression of CYP3A7 was slightly increased in HBV cirrhosis. The expression of CYP4A11 was decreased 85% in HCC, 7% in alcoholic cirrhosis and severe liver cirrhosis, along with decreases in PPAR?. The 93 end-stage livers had much higher inter-individual variations in gene expression than 35 normal livers.The expression of CYP enzyme genes and corresponding nuclear receptors was generally decreased in end-stage liver diseases, and significant differences in gene expression were evident between peri-HCC and HCC.

SUBMITTER: Chen H

PROVIDER: S-EPMC4093721 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Expression of P450 and nuclear receptors in normal and end-stage Chinese livers.

Chen Hong H Shen Zhong-Yang ZY Xu Wang W Fan Tie-Yan TY Li Jun J Lu Yuan-Fu YF Cheng Ming-Liang ML Liu Jie J

World journal of gastroenterology 20140701 26

<h4>Aim</h4>To investigate the expression of P450 enzyme genes by using end-stage liver disease samples and trimmed normal Chinese donor livers.<h4>Methods</h4>The end-stage liver disease samples [n = 93, including hepatocellular carcinoma (HCC), peri-HCC tissue, hepatitis B virus cirrhosis, alcoholic cirrhosis, and severe cirrhosis] and trimmed normal Chinese donor livers (n = 35) from The Institute of Organ Transplantation in Beijing, China. Total RNA was extracted, purified, and subjected to ...[more]

PMID: 25024626

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Dataset Information

Expression of P450 and nuclear receptors in normal and end-stage Chinese livers.

Publications

Expression of P450 and nuclear receptors in normal and end-stage Chinese livers.

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