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Determinants for membrane association of the hepatitis C virus NS2 protease domain.


ABSTRACT: Hepatitis C virus (HCV) nonstructural protein 2 (NS2) is required for HCV polyprotein processing and particle assembly. It comprises an N-terminal membrane domain and a C-terminal, cytosolically oriented protease domain. Here, we demonstrate that the NS2 protease domain itself associates with cellular membranes. A single charged residue in the second ?-helix of the NS2 protease domain is required for proper membrane association, NS2 protein stability, and efficient HCV polyprotein processing.

SUBMITTER: Lange CM 

PROVIDER: S-EPMC4093864 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Determinants for membrane association of the hepatitis C virus NS2 protease domain.

Lange Christian M CM   Bellecave Pantxika P   Dao Thi Viet Loan VL   Tran Huong T L HT   Penin François F   Moradpour Darius D   Gouttenoire Jérôme J  

Journal of virology 20140319 11


Hepatitis C virus (HCV) nonstructural protein 2 (NS2) is required for HCV polyprotein processing and particle assembly. It comprises an N-terminal membrane domain and a C-terminal, cytosolically oriented protease domain. Here, we demonstrate that the NS2 protease domain itself associates with cellular membranes. A single charged residue in the second α-helix of the NS2 protease domain is required for proper membrane association, NS2 protein stability, and efficient HCV polyprotein processing. ...[more]

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