Overcoming preexisting humoral immunity to AAV using capsid decoys.
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ABSTRACT:
SUBMITTER: Mingozzi F
PROVIDER: S-EPMC4095828 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Publications
Overcoming preexisting humoral immunity to AAV using capsid decoys.
Mingozzi Federico F Anguela Xavier M XM Pavani Giulia G Chen Yifeng Y Davidson Robert J RJ Hui Daniel J DJ Yazicioglu Mustafa M Elkouby Liron L Hinderer Christian J CJ Faella Armida A Howard Carolann C Tai Alex A Podsakoff Gregory M GM Zhou Shangzhen S Basner-Tschakarjan Etiena E Wright John Fraser JF High Katherine A KA
Science translational medicine 20130701 194
Adeno-associated virus (AAV) vectors delivered through the systemic circulation successfully transduce various target tissues in animal models. However, similar attempts in humans have been hampered by the high prevalence of neutralizing antibodies to AAV, which completely block vector transduction. We show in both mouse and nonhuman primate models that addition of empty capsid to the final vector formulation can, in a dose-dependent manner, adsorb these antibodies, even at high titers, thus ove ...[more]
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