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SSFinder: high throughput CRISPR-Cas target sites prediction tool.

ABSTRACT: Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with Windows, Mac OS, and Linux operating systems, and freely available online.

SUBMITTER: Upadhyay SK

PROVIDER: S-EPMC4095993 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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SSFinder: high throughput CRISPR-Cas target sites prediction tool.

Upadhyay Santosh Kumar SK Upadhyay Santosh Kumar SK Sharma Shailesh S

BioMed research international 20140626

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with W ...[more]

PMID: 25089276

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