In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish.
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ABSTRACT:
SUBMITTER: Langenau DM
PROVIDER: S-EPMC409925 | biostudies-literature | 2004 May
REPOSITORIES: biostudies-literature
Publications
In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish.
Langenau David M DM Ferrando Adolfo A AA Traver David D Kutok Jeffery L JL Hezel John-Paul D JP Kanki John P JP Zon Leonard I LI Look A Thomas AT Trede Nikolaus S NS
Proceedings of the National Academy of Sciences of the United States of America 20040503 19
Transgenic zebrafish that express GFP under control of the T cell-specific tyrosine kinase (lck) promoter were used to analyze critical aspects of the immune system, including patterns of T cell development and T cell homing after transplant. GFP-labeled T cells could be ablated in larvae by either irradiation or dexamethasone added to the water, illustrating that T cells have evolutionarily conserved responses to chemical and radiation ablation. In transplant experiments, thymocytes from lck-GF ...[more]
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