Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

ABSTRACT: High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ?700 ?m × 700 ?m × 200 ?m at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

SUBMITTER: Prevedel R 

PROVIDER: S-EPMC4100252 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature