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Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

ABSTRACT: High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ?700 ?m × 700 ?m × 200 ?m at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

SUBMITTER: Prevedel R

PROVIDER: S-EPMC4100252 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

Prevedel Robert R Yoon Young-Gyu YG Hoffmann Maximilian M Pak Nikita N Wetzstein Gordon G Kato Saul S Schrödel Tina T Raskar Ramesh R Zimmer Manuel M Boyden Edward S ES Vaziri Alipasha A

Nature methods 20140518 7

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging. ...[more]

PMID: 24836920

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