Improved method of plasma 8-Isoprostane measurement and association analyses with habitual drinking and smoking.

ABSTRACT:

Aim

To develop a simple and accurate method for quantifying 8-isoprostane in plasma by employing a combination of two-step solid-phase extraction of samples and a commercially available ELISA kit, and by this method to examine the effects of drinking and smoking habits against the levels of plasma 8-isoprostane in healthy Japanese volunteers.

Methods

Plasma 8-isoprostane was extracted with ODS gel suspension followed by NH(2) Sep-Pak column. The 8-isoprostane fractions were assayed using a commercially available ELISA kit. We measured plasma 8-isoprostane levels in 157 healthy Japanese volunteers divided into three groups (64 non-habitual drinkers, 56 moderate drinkers and 37 habitual drinkers) according to their alcohol consumption per week. Genotypes of aldehyde dehydrogen

SUBMITTER: Kitano S 

PROVIDER: S-EPMC4100666 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature