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MMP-14 is necessary but not sufficient for invasion of three-dimensional collagen by human muscle satellite cells.

ABSTRACT: The twenty-five known matrix metalloproteases (MMPs) and their endogenous inhibitors, tissue inhibitors of metalloproteases (TIMPs), mediate cell invasion through the extracellular matrix (ECM). In a comparative three-dimensional assay, we analyzed human and mouse satellite cells' competence to invade an artificial ECM (collagen I). We identified a single MMP that 1) is expressed by human muscle satellite cells; 2) is induced at the mRNA/protein level by adhesion to collagen I; and 3) is necessary for invasion into a collagen I matrix. Interestingly, murine satellite cells neither express this MMP, nor invade the collagen matrix. However, exogenous human MMP-14 is not sufficient to induce invasion of a collagen matrix by murine cells, emphasizing species differences.

SUBMITTER: Lund DK

PROVIDER: S-EPMC4101619 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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MMP-14 is necessary but not sufficient for invasion of three-dimensional collagen by human muscle satellite cells.

Lund Dane K DK Mouly Vincent V Cornelison D D W DD

American journal of physiology. Cell physiology 20140604 2

The twenty-five known matrix metalloproteases (MMPs) and their endogenous inhibitors, tissue inhibitors of metalloproteases (TIMPs), mediate cell invasion through the extracellular matrix (ECM). In a comparative three-dimensional assay, we analyzed human and mouse satellite cells' competence to invade an artificial ECM (collagen I). We identified a single MMP that 1) is expressed by human muscle satellite cells; 2) is induced at the mRNA/protein level by adhesion to collagen I; and 3) is necessa ...[more]

PMID: 24898588

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