Dataset Information

Development and testing of new gene-homologous EST-SSRs for Eucalyptus gomphocephala (Myrtaceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:New microsatellite (simple sequence repeat [SSR]) primers were developed from Eucalyptus expressed sequence tags (ESTs) and optimized for genetic studies of the southwestern Australian tree E. gomphocephala, which is severely impacted by tree health decline and habitat fragmentation. • METHODS AND RESULTS:A total of 133 gene-homologous EST-SSR primer pairs were designed for Eucalyptus, and 44 were screened in E. gomphocephala. Of these, 17 produced reliable amplification products and 11 were polymorphic. Between two and 13 alleles were observed per locus, and observed heterozygosities ranged from 0.172 to 0.867. All 17 EST-SSRs that amplified E. gomphocephala cross-amplified to at least one of E. marginata, E. camaldulensis, and E. victrix. • CONCLUSIONS:This set of EST-SSR primer pairs will be valuable tools for future population genetic studies of E. gomphocephala and other eucalypts, particularly for studying gene-linked variation and informing seed-sourcing strategies for ecological restoration.

SUBMITTER: Bradbury D

PROVIDER: S-EPMC4103447 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Development and testing of new gene-homologous EST-SSRs for Eucalyptus gomphocephala (Myrtaceae).

Bradbury Donna D Smithson Ann A Krauss Siegfried L SL

Applications in plant sciences 20130722 8

<h4>Unlabelled</h4><h4>Premise of the study</h4>New microsatellite (simple sequence repeat [SSR]) primers were developed from Eucalyptus expressed sequence tags (ESTs) and optimized for genetic studies of the southwestern Australian tree E. gomphocephala, which is severely impacted by tree health decline and habitat fragmentation. •<h4>Methods and results</h4>A total of 133 gene-homologous EST-SSR primer pairs were designed for Eucalyptus, and 44 were screened in E. gomphocephala. Of these, 17 p ...[more]

PMID: 25202574

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Project description:Salt stress adversely affects the global wheat production and productivity. To improve salinity tolerance of crops, identification of robust molecular markers is highly imperative for development of salt-tolerant cultivars to mimic yield losses under saline conditions. In this study, we mined 171 salt-responsive genes (including 10 miRNAs) from bread wheat genome using the sequence information of functionally validated salt-responsive rice genes. Salt-stress, tissue and developmental stage-specific expression analysis of RNA-seq datasets revealed the constitutive as well as the inductive response of salt-responsive genes in different tissues of wheat. Fifty-four genotypes were phenotyped for salt stress tolerance. The stress tolerance index of the genotypes ranged from 0.30 to 3.18. In order to understand the genetic diversity, candidate gene based SSRs (cg-SSRs) and MIR gene based SSRs (miR-SSRs) were mined from 171 members of salt-responsive genes of wheat and validated among the contrasting panels of 54 tolerant as well as susceptible wheat genotypes. Among 53 SSR markers screened, 10 cg-SSRs and 8 miR-SSRs were found to be polymorphic. Polymorphic information content between the wheat genotypes ranged from 0.07 to 0.67, indicating the extant of wide genetic variation among the salt tolerant and susceptible genotypes at the DNA level. The genetic diversity analysis based on the allelic data grouped the wheat genotypes into three separate clusters of which single group encompassing most of the salt susceptible genotypes and two of them containing salt tolerance and moderately salt tolerance wheat genotypes were in congruence with penotypic data. Our study showed that both salt-responsive genes and miRNAs based SSRs were more diverse and can be effectively used for diversity analysis. This study reports the first extensive survey on genome-wide analysis, identification, development and validation of salt-responsive cg-SSRs and miR-SSRs in wheat. The information generated in the present study on genetic divergence among genotypes having a differential response to salt will help in the selection of suitable lines as parents for developing salt tolerant cultivars in wheat.

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Dataset Information

Development and testing of new gene-homologous EST-SSRs for Eucalyptus gomphocephala (Myrtaceae).

Publications

Development and testing of new gene-homologous EST-SSRs for Eucalyptus gomphocephala (Myrtaceae).

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