Dataset Information

Development and characterization of microsatellite primers in Geranium carolinianum (Geraniaceae) with 454 sequencing.

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite primers were developed for Geranium carolinanum, a North American winter annual herb, for use in population genetic analyses. • METHODS AND RESULTS:Genomic DNA enriched for repeat-containing fragments was sequenced on the Roche 454 Titanium platform, resulting in 470 primer pairs developed from 1115 microsatellite-containing sequences. A subset of 37 primer pairs was screened for polymorphism across three native and three invasive populations. We identified four monomorphic and eight polymorphic loci. Polymorphic loci contained between two and seven alleles per locus, and mean within-population expected heterozygosity ranged from 0.100 to 0.290. Within populations, observed heterozygosity for individual loci ranged from zero to 0.857, and expected heterozygosity ranged from 0.046 to 0.559. • CONCLUSIONS:These microsatellite markers will be useful for future studies of genetic diversity, structure, and mating systems across the geographic range of G. carolinianum, and may be transferable to other closely related species.

SUBMITTER: Shirk RY

PROVIDER: S-EPMC4103449 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Development and characterization of microsatellite primers in Geranium carolinianum (Geraniaceae) with 454 sequencing.

Shirk Rebecca Y RY Glenn Travis C TC Chang Shu-Mei SM Hamrick J L JL

Applications in plant sciences 20130722 8

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite primers were developed for Geranium carolinanum, a North American winter annual herb, for use in population genetic analyses. •<h4>Methods and results</h4>Genomic DNA enriched for repeat-containing fragments was sequenced on the Roche 454 Titanium platform, resulting in 470 primer pairs developed from 1115 microsatellite-containing sequences. A subset of 37 primer pairs was screened for polymorphism across three native and three inva ...[more]

PMID: 25202576

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Project description:BACKGROUND AND AIMS: Physical dormancy (PY)-break in some annual plant species is a two-step process controlled by two different temperature and/or moisture regimes. The thermal time model has been used to quantify PY-break in several species of Fabaceae, but not to describe stepwise PY-break. The primary aims of this study were to quantify the thermal requirement for sensitivity induction by developing a thermal time model and to propose a mechanism for stepwise PY-breaking in the winter annual Geranium carolinianum. METHODS: Seeds of G. carolinianum were stored under dry conditions at different constant and alternating temperatures to induce sensitivity (step I). Sensitivity induction was analysed based on the thermal time approach using the Gompertz function. The effect of temperature on step II was studied by incubating sensitive seeds at low temperatures. Scanning electron microscopy, penetrometer techniques, and different humidity levels and temperatures were used to explain the mechanism of stepwise PY-break. KEY RESULTS: The base temperature (Tb) for sensitivity induction was 17·2 °C and constant for all seed fractions of the population. Thermal time for sensitivity induction during step I in the PY-breaking process agreed with the three-parameter Gompertz model. Step II (PY-break) did not agree with the thermal time concept. Q10 values for the rate of sensitivity induction and PY-break were between 2·0 and 3·5 and between 0·02 and 0·1, respectively. The force required to separate the water gap palisade layer from the sub-palisade layer was significantly reduced after sensitivity induction. CONCLUSIONS: Step I and step II in PY-breaking of G. carolinianum are controlled by chemical and physical processes, respectively. This study indicates the feasibility of applying the developed thermal time model to predict or manipulate sensitivity induction in seeds with two-step PY-breaking processes. The model is the first and most detailed one yet developed for sensitivity induction in PY-break.

Dataset Information

Development and characterization of microsatellite primers in Geranium carolinianum (Geraniaceae) with 454 sequencing.

Publications

Development and characterization of microsatellite primers in Geranium carolinianum (Geraniaceae) with 454 sequencing.

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