Dataset Information

Microsatellite primers in the weedy annual herb Anacyclus clavatus (Asteraceae) and four closely related species.

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Nuclear microsatellite primers were developed for the weedy herb Anacyclus clavatus to study the genetic structure of hybrid zones with closely related taxa in the western Mediterranean Basin, where different floral phenotypes are present. • METHODS AND RESULTS:We obtained two microsatellite libraries using next-generation sequencing and Sanger sequencing of cloned restriction fragments. A total of 13 polymorphic and 11 monomorphic loci were identified in three Iberian populations of A. clavatus. The primers amplified di- and trinucleotide repeats with 1-8 alleles per locus. Most primers also amplified in A. homogamos, A. monanthos, A. radiatus, and A. valentinus. • CONCLUSIONS:These results indicate the utility of these markers in A. clavatus for population genetic and hybridization studies as well as their applicability across the genus.

SUBMITTER: Agudo A

PROVIDER: S-EPMC4103460 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Microsatellite primers in the weedy annual herb Anacyclus clavatus (Asteraceae) and four closely related species.

Agudo Alicia A Picó F Xavier FX Alvarez Inés I

Applications in plant sciences 20131031 11

<h4>Unlabelled</h4><h4>Premise of the study</h4>Nuclear microsatellite primers were developed for the weedy herb Anacyclus clavatus to study the genetic structure of hybrid zones with closely related taxa in the western Mediterranean Basin, where different floral phenotypes are present. •<h4>Methods and results</h4>We obtained two microsatellite libraries using next-generation sequencing and Sanger sequencing of cloned restriction fragments. A total of 13 polymorphic and 11 monomorphic loci were ...[more]

PMID: 25202498

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Project description:Background and Aims:Interstitial telomeric repeat (ITR) sites, consisting of tandem repeats of telomeric motifs localized at intrachromosomal sites, have been reported in a few unrelated organisms including plants. However, the causes for the occurrence of ITRs outside of the chromosomal termini are not fully understood. One possible explanation are the chromosomal rearrangements involving telomeric sites, which could also affect the location of other structural genome elements, such as the 45S rDNA. Taking advantage of the high dynamism in 45S rDNA loci previously found in Anacyclus (Asteraceae, Anthemideae), the occurrence and patterns of variation of ITRs were explored in this genus with the aim of finding common underlying causes. Methods:In total, 132 individuals from 44 populations of nine species were analysed by fluorescence in situ hybridization using an Arabidopsis-type telomeric sequence as a probe. Key results:Variable presence of ITR sites was detected in six out of nine species of Anacyclus, ranging from two to 45 sites and showing contrasting chromosomal locations and a differential presence of the ITR site on homologous chromosome pairs. At the intraspecific level, the ranges were as large as 0-12 ITR sites. Although only 26 % of the total observed ITR sites were localized in chromosomes bearing 45S rDNA loci, all cases of interstitial 45S rDNA reported in a previous work co-occurred with ITRs in close proximity in the same chromosome arms. Conclusions:High levels of ITR polymorphism within a single species have not been previously reported in plants and suggest that this pattern might have been overlooked due to insufficient sampling. Although ancient Robertsonian translocations or the amplification of terminal 45S rDNA sites cannot, on their own, explain all of the levels of variability in ITRs reported here, there are suggestions that they may have been involved in the evolutionary history of this genus or its ancestors in Anthemideae.

Dataset Information

Microsatellite primers in the weedy annual herb Anacyclus clavatus (Asteraceae) and four closely related species.

Publications

Microsatellite primers in the weedy annual herb Anacyclus clavatus (Asteraceae) and four closely related species.

Similar Datasets

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