Browse
Submit Data
Databases
API
Help

Dataset Information

22 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Development and characterization of polymorphic microsatellite loci in Phellodendron amurense (Rutaceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite markers were developed for the rare species Phellodendron amurense to assess the genetic diversity and population structure of this plant. • METHODS AND RESULTS:In total, 27 microsatellite markers were developed for P. amurense by using an enriched genomic library and hybridization; all of these primers successfully amplified DNA fragments in P. amurense. These markers were screened in 74 individuals from four populations in China; 15 loci were found to be polymorphic, with the number of alleles per locus ranging from one to nine. • CONCLUSIONS:The microsatellite markers developed here represent a useful tool for studying the population genetic structure of P. amurense and to inform toward the development of effective conservation programs for this species.

SUBMITTER: Yu JH

PROVIDER: S-EPMC4105284 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae).

Project description:Saxifraga egregia (Saxifragaceae) is a perennial herb that is endemic to the Qinghai-Tibet Plateau. We developed 12 polymorphic microsatellite loci for S. egregia to investigate its population genetics. Forty-eight pairs of microsatellite primers (including 36 monomorphic loci) were isolated and characterized by magnetic bead enrichment. Twelve of these markers showed polymorphism, and the number of alleles per locus ranged from four to 14 across 50 individuals from three populations of S. egregia. No linkage disequilibrium was detected in any pair of loci. These polymorphic markers are expected to be helpful in further studies on the systematics and phylogeography of S. egregia in the Qinghai-Tibet Plateau.

| S-EPMC4542942 | biostudies-literature

Phellodendron amurense

Project description:Phellodendron amurense Raw sequence reads

| PRJNA859281 | ENA

Phellodendron amurense

Project description:Phellodendron amurense Transcriptome or Gene expression

| PRJNA213663 | ENA

Phellodendron amurense

Project description:Phellodendron amurense overview

| PRJNA400094 | ENA

Phellodendron amurense

Project description:Phellodendron amurense Genome sequencing and assembly

| PRJNA787723 | ENA

Phellodendron amurense

Project description:Chromosome-level genome assembly of Phellodendron amurense

| PRJNA1184009 | ENA

Phellodendron amurense

Project description:Phellodendron amurense Raw sequence reads

| PRJNA932051 | ENA

Phellodendron amurense

Project description:Transcriptome of stem development in Phellodendron amurense

| PRJNA817294 | ENA

Development and characterization of microsatellite primers for Zanthoxylum schinifolium (Rutaceae).

Project description:PREMISE OF THE STUDY:Polymorphic microsatellite markers of Zanthoxylum schinifolium (Rutaceae), a promising medicinal plant with effective antibacterial, anticancer, and anti-inflammatory compounds, were developed and evaluated for further genetic studies based on genetic variation among individuals or populations. METHODS AND RESULTS:Following the selective hybridization method, microsatellite-enrichment libraries were constructed. Using these libraries, we obtained 15 polymorphic and three monomorphic microsatellite markers for Z. schinifolium. The number of alleles observed in each of the 15 polymorphic loci ranged from two to eight, and the observed and expected heterozygosities ranged from 0.070 to 0.677 and from 0.093 to 0.688, respectively. Eleven of these developed markers were successfully amplified for Z. piperitum, a related species. CONCLUSIONS:These microsatellite markers can be valuable tools for further genetic studies of Z. schinifolium, such as genetic resource conservation for maintaining breeding material and individual identification for breeding program improvement and variety management.

| S-EPMC5546163 | biostudies-literature

Development of 14 polymorphic microsatellite loci for Ficus tikoua (Moraceae).

Project description:Polymorphic microsatellite markers were developed to facilitate studies on the fine-scale population genetic structure of Ficus tikoua (Moraceae), a prostrate shrub known to have highly restricted gene flow. Microsatellite primers were developed using the biotin-streptavidin capture method and scanned for polymorphism within 76 individuals sampled from three natural F. tikoua populations. Fourteen loci were shown to be polymorphic, with allele numbers ranging from three to 16. The observed and expected heterozygosity in the three populations ranged from 0 to 1 and from 0 to 0.87, respectively. Substantial divergence was found among the populations at some loci. All loci can be successfully amplified in at least eight other Ficus species, indicating good transferability within the genus. The 14 microsatellite loci will be a helpful tool for assessing the fine-scale genetic structure of F. tikoua.

| S-EPMC4795916 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data