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Characterization of microsatellite loci in Tilia platyphyllos (Malvaceae) and cross-amplification in related species.

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite markers in the genus Tilia were developed to investigate the genetic variation in T. platyphyllos and its relationship with T. cordata. • METHODS AND RESULTS:Fifteen microsatellite markers were developed using a microsatellite enrichment protocol. Most loci show a high level of polymorphism in two T. platyphyllos populations from France. The number of alleles ranged from one to 15, with a mean of 8.96. The mean observed and expected heterozygosities were 0.71 and 0.70, respectively. Cross-amplification results indicated that 12 out of 15 loci amplified polymorphic loci in 23 species in the genus. • CONCLUSIONS:These markers will be useful tools for investigating the phylogeography and hybridization of Tilia species.

SUBMITTER: Phuekvilai P

PROVIDER: S-EPMC4105290 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Characterization of microsatellite loci in Tilia platyphyllos (Malvaceae) and cross-amplification in related species.

Phuekvilai Prattana P Wolff Kirsten K

Applications in plant sciences 20130328 4

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite markers in the genus Tilia were developed to investigate the genetic variation in T. platyphyllos and its relationship with T. cordata. •<h4>Methods and results</h4>Fifteen microsatellite markers were developed using a microsatellite enrichment protocol. Most loci show a high level of polymorphism in two T. platyphyllos populations from France. The number of alleles ranged from one to 15, with a mean of 8.96. The mean observed and ex ...[more]

PMID: 25202533

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