Ontology highlight
ABSTRACT:
SUBMITTER: Wu Y
PROVIDER: S-EPMC4105320 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Wu Yicong Y Wawrzusin Peter P Senseney Justin J Fischer Robert S RS Christensen Ryan R Santella Anthony A York Andrew G AG Winter Peter W PW Waterman Clare M CM Bao Zhirong Z Colón-Ramos Daniel A DA McAuliffe Matthew M Shroff Hari H
Nature biotechnology 20131013 11
Optimal four-dimensional imaging requires high spatial resolution in all dimensions, high speed and minimal photobleaching and damage. We developed a dual-view, plane illumination microscope with improved spatiotemporal resolution by switching illumination and detection between two perpendicular objectives in an alternating duty cycle. Computationally fusing the resulting volumetric views provides an isotropic resolution of 330 nm. As the sample is stationary and only two views are required, we ...[more]