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Luteal activity of pregnant rats with hypo-and hyperthyroidism.


ABSTRACT: BACKGROUND: Luteal activity is dependent on the interaction of various growth factors, cytokines and hormones, including the thyroid hormones, being that hypo- and hyperthyroidism alter the gestational period and are also a cause of miscarriage and stillbirth. Because of that, we evaluated the proliferation, apoptosis and expression of angiogenic factors and COX-2 in the corpus luteum of hypo- and hyperthyroid pregnant rats. METHODS: Seventy-two adult female rats were equally distributed into three groups: hypothyroid, hyperthyroid and control. Hypo- and hyperthyroidism were induced by the daily administration of propylthiouracil and L-thyroxine, respectively. The administration began five days before becoming pregnant and the animals were sacrificed at days 10, 14, and 19 of gestation. We performed an immunohistochemical analysis to evaluate the expression of CDC-47, VEGF, Flk-1 (VEGF receptor) and COX-2. Apoptosis was evaluated by the TUNEL assay. We assessed the gene expression of VEGF, Flk-1, caspase 3, COX-2 and PGF2? receptor using real time RT-PCR. The data were analyzed by SNK test. RESULTS: Hypothyroidism reduced COX-2 expression on day 10 and 19 (P?

SUBMITTER: Silva JF 

PROVIDER: S-EPMC4107585 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Luteal activity of pregnant rats with hypo-and hyperthyroidism.

Silva Juneo Freitas JF   Ocarino Natália Melo NM   Serakides Rogéria R  

Journal of ovarian research 20140712


<h4>Background</h4>Luteal activity is dependent on the interaction of various growth factors, cytokines and hormones, including the thyroid hormones, being that hypo- and hyperthyroidism alter the gestational period and are also a cause of miscarriage and stillbirth. Because of that, we evaluated the proliferation, apoptosis and expression of angiogenic factors and COX-2 in the corpus luteum of hypo- and hyperthyroid pregnant rats.<h4>Methods</h4>Seventy-two adult female rats were equally distri  ...[more]

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