Dataset Information

Periaortic brown adipose tissue as a major determinant of [¹?F]-fluorodeoxyglucose vascular uptake in atherosclerosis-prone, apoE-/- mice.

ABSTRACT:

Background

[18F]-fluorodeoxyglucose (FDG) has been suggested for the clinical and experimental imaging of inflammatory atherosclerotic lesions. Significant FDG uptake in brown adipose tissue (BAT) has been observed both in humans and mice. The objective of the present study was to investigate the influence of periaortic BAT on apolipoprotein E-deficient (apoE-/-) mouse atherosclerotic lesion imaging with FDG.

Methods

ApoE-/- mice (36 ± 2 weeks-old) were injected with FDG (12 ± 2 MBq). Control animals (Group A, n?=?7) were injected conscious and kept awake at room temperature (24°C) throughout the accumulation period. In order to minimize tracer activity in periaortic BAT, Group B (n?=?7) and C (n?=?6) animals were injected under anaesthesia at 37°C and Group C animals were additionally pre-treated with propranolol. PET/CT acquisitions were performed prior to animal euthanasia and ex vivo analysis of FDG biodistribution.

Results

Autoradiographic imaging indicated higher FDG uptake in atherosclerotic lesions than in the normal aortic wall (all groups, P<0.05) and the blood (all groups, P<0.01) which correlated with macrophage infiltration (R?=?0.47; P<0.001). However, periaortic BAT uptake was either significantly higher (Group A, P<0.05) or similar (Group B and C, P?=?NS) to that observed in atherosclerotic lesions and was shown to correlate with in vivo quantified aortic FDG activity.

Conclusion

Periaortic BAT FDG uptake was identified as a confounding factor while using FDG for the non-invasive imaging of mouse atherosclerotic lesions.

SUBMITTER: Toczek J

PROVIDER: S-EPMC4108473 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Periaortic brown adipose tissue as a major determinant of [¹⁸F]-fluorodeoxyglucose vascular uptake in atherosclerosis-prone, apoE-/- mice.

Toczek Jakub J Broisat Alexis A Perret Pascale P Desruet Marie-Dominique MD Fagret Daniel D Riou Laurent M LM Ghezzi Catherine C

PloS one 20140723 7

<h4>Background</h4>[18F]-fluorodeoxyglucose (FDG) has been suggested for the clinical and experimental imaging of inflammatory atherosclerotic lesions. Significant FDG uptake in brown adipose tissue (BAT) has been observed both in humans and mice. The objective of the present study was to investigate the influence of periaortic BAT on apolipoprotein E-deficient (apoE-/-) mouse atherosclerotic lesion imaging with FDG.<h4>Methods</h4>ApoE-/- mice (36 ± 2 weeks-old) were injected with FDG (12 ± 2 M ...[more]

PMID: 25054923

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