Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.

ABSTRACT: Human stem cell factor initiates a diverse array of cellular responses, including hematopoiesis, cell proliferation, differentiation, migration and survival. To explore the relationship between its structure and function, we produced recombinant soluble human stem cell factor1-165 (wild type) and human stem cell factor1-141 (C-terminal truncated) in a yeast expression system and compared their biological activities and thermal stabilities. The biological activity of the two proteins was measured as a function of TF-1 cell viability and effects on downstream signaling targets after incubation. We found that these proteins enhanced cell viability and downstream signaling to a similar extent, in a dose-dependent manner. The biological activity of recombinant human stem cell factor1-165 was si

SUBMITTER: Weng YP 

PROVIDER: S-EPMC4111497 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature