Applying thiouracil tagging to mouse transcriptome analysis.
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ABSTRACT:
SUBMITTER: Gay L
PROVIDER: S-EPMC4112099 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Publications
Applying thiouracil tagging to mouse transcriptome analysis.
Gay Leslie L Karfilis Kate V KV Miller Michael R MR Doe Chris Q CQ Stankunas Kryn K
Nature protocols 20140123 2
Transcriptional profiling is a powerful approach for studying mouse development, physiology and disease models. Here we describe a protocol for mouse thiouracil tagging (TU tagging), a transcriptome analysis technology that includes in vivo covalent labeling, purification and analysis of cell type-specific RNA. TU tagging enables the isolation of RNA from a given cell population of a complex tissue, avoiding transcriptional changes induced by cell isolation trauma, as well as the identification ...[more]
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