KIF5B-RET fusion kinase promotes cell growth by multilevel activation of STAT3 in lung cancer.

ABSTRACT:

Background

Lung cancer in nonsmokers tends to be driven by a single somatic mutation or a gene fusion. KIF5B-RET fusion is an oncogene identified in non-small cell lung cancers. In this study, we verified the oncogenic activity of KIF5B-RET fusion and investigated how KIF5B-RET activates the specific signaling pathways for cellular transformation. We aimed to provide a basis for the further development of the therapy for KIF5B-RET positive lung cancer patients.

Methods

RT-PCR was used to screen for KIF5B-RET fusions in Chinese lung cancer patients. To verify the oncogenic activity of KIF5B-RET kinase in lung cancer cells, we manipulated its expression genetically followed by colony formation and tumor formation assays. The mechanism by which KIF5B-RET kinase induces prolifer

SUBMITTER: Qian Y 

PROVIDER: S-EPMC4114102 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature