Unknown

Dataset Information

0

A possible mechanism for redox control of human neuroglobin activity.


ABSTRACT: Neuroglobin (Ngb) promotes neuron survival under hypoxic/ischemic conditions. In vivo and in vitro assays provide evidence for redox-regulated functioning of Ngb. On the basis of X-ray crystal structures and our MD simulations, a mechanism for redox control of human Ngb (hNgb) activity via the influence of the CD loop on the active site is proposed. We provide evidence that the CD loop undergoes a strand-to-helix transition when the external environment becomes sufficiently oxidizing, and that this CD loop conformational transition causes critical restructuring of the active site. We postulate that the strand-to-helix mechanics of the CD loop allows hNgb to utilize the lability of Cys46/Cys55 disulfide bonding and of the Tyr44/His64/heme propionate interaction network for redox-controlled functioning of hNgb.

SUBMITTER: Morozov AN 

PROVIDER: S-EPMC4114473 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

A possible mechanism for redox control of human neuroglobin activity.

Morozov Alexander N AN   Roach James P JP   Kotzer Margarita M   Chatfield David C DC  

Journal of chemical information and modeling 20140619 7


Neuroglobin (Ngb) promotes neuron survival under hypoxic/ischemic conditions. In vivo and in vitro assays provide evidence for redox-regulated functioning of Ngb. On the basis of X-ray crystal structures and our MD simulations, a mechanism for redox control of human Ngb (hNgb) activity via the influence of the CD loop on the active site is proposed. We provide evidence that the CD loop undergoes a strand-to-helix transition when the external environment becomes sufficiently oxidizing, and that t  ...[more]

Similar Datasets

| S-EPMC3093900 | biostudies-literature
| S-EPMC9062163 | biostudies-literature
| S-EPMC536024 | biostudies-literature
| S-EPMC8393432 | biostudies-literature
| S-EPMC5818181 | biostudies-literature
| S-EPMC8159454 | biostudies-literature
| S-EPMC4736404 | biostudies-other
| S-EPMC8699588 | biostudies-literature
2016-12-31 | GSE74797 | GEO
| S-EPMC4238221 | biostudies-literature