Ontology highlight
ABSTRACT:
SUBMITTER: Bothma JP
PROVIDER: S-EPMC4115566 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Bothma Jacques P JP Garcia Hernan G HG Esposito Emilia E Schlissel Gavin G Gregor Thomas T Levine Michael M
Proceedings of the National Academy of Sciences of the United States of America 20140703 29
We present the use of recently developed live imaging methods to examine the dynamic regulation of even-skipped (eve) stripe 2 expression in the precellular Drosophila embryo. Nascent transcripts were visualized via MS2 RNA stem loops. The eve stripe 2 transgene exhibits a highly dynamic pattern of de novo transcription, beginning with a broad domain of expression during nuclear cycle 12 (nc12), and progressive refinement during nc13 and nc14. The mature stripe 2 pattern is surprisingly transien ...[more]