Ontology highlight
ABSTRACT:
SUBMITTER: Zhong R
PROVIDER: S-EPMC4117740 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Zhong Rui R Kim Jimi J Kim Hyun Seok HS Kim Minsoo M Lum Lawrence L Levine Beth B Xiao Guanghua G White Michael A MA Xie Yang Y
Nucleic acids research 20140627 13
A challenge for large-scale siRNA loss-of-function studies is the biological pleiotropy resulting from multiple modes of action of siRNA reagents. A major confounding feature of these reagents is the microRNA-like translational quelling resulting from short regions of oligonucleotide complementarity to many different messenger RNAs. We developed a computational approach, deconvolution analysis of RNAi screening data, for automated quantitation of off-target effects in RNAi screening data sets. S ...[more]