Ontology highlight
ABSTRACT:
SUBMITTER: Paul R
PROVIDER: S-EPMC4118813 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Acta crystallographica. Section F, Structural biology communications 20140723 Pt 8
Ribokinase (RK) is one of the principal enzymes in carbohydrate metabolism, catalyzing the reaction of D-ribose and adenosine triphosphate to produce ribose-5-phosphate and adenosine diphosphate (ADP). To provide further insight into the catalytic mechanism, the rbsK gene from Vibrio cholerae O395 encoding ribokinase was cloned and the protein was overexpressed in Escherichia coli BL21 (DE3) and purified using Ni(2+)-NTA affinity chromatography. Crystals of V. cholerae RK (Vc-RK) and of its comp ...[more]