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Geodermatophilus poikilotrophi sp. nov.: a multitolerant actinomycete isolated from dolomitic marble.

ABSTRACT: A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18T, was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15-35°C, at pH 5.5-9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0 and the unsaturated C17:1 ?8c and C16:1 ?7c. The 16S rRNA gene showed 97.4-99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18T is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18T (=DSM 44209T=CCUG 63018T). The INSDC accession number is HF970583. The novel R software package lethal was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.

SUBMITTER: del Carmen Montero-Calasanz M

PROVIDER: S-EPMC4119925 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Geodermatophilus poikilotrophi sp. nov.: a multitolerant actinomycete isolated from dolomitic marble.

del Carmen Montero-Calasanz Maria M Hofner Benjamin B Göker Markus M Rohde Manfred M Spröer Cathrin C Hezbri Karima K Gtari Maher M Schumann Peter P Klenk Hans-Peter HP

BioMed research international 20140709

A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18T, was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15-35°C, at pH 5.5-9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for othe ...[more]

PMID: 25114928

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Dataset Information

Geodermatophilus poikilotrophi sp. nov.: a multitolerant actinomycete isolated from dolomitic marble.

Publications

Geodermatophilus poikilotrophi sp. nov.: a multitolerant actinomycete isolated from dolomitic marble.

Similar Datasets

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