Dataset Information

Motility efficiency and spatiotemporal synchronization in non-metastatic vs. metastatic breast cancer cells.

ABSTRACT: Metastatic breast cancer cells move not only more rapidly and persistently than their non-metastatic variants but in doing so use the mechanical work of the cytoskeleton more efficiently. The efficiency of the cell motions is defined for entire cells (rather than parts of the cell membrane) and is related to the work expended in forming membrane protrusions and retractions. This work, in turn, is estimated by integrating the protruded and retracted areas along the entire cell perimeter and is standardized with respect to the net translocation of the cell. A combination of cross-correlation, Granger causality, and morphodynamic profiling analyses is then used to relate the efficiency to the cell membrane dynamics. In metastatic cells, the protrusions and retractions are highly "synchronized" both in space and in time and these cells move efficiently. In contrast, protrusions and retractions formed by non-metastatic cells are not "synchronized" corresponding to low motility efficiencies. Our work provides a link between the kinematics of cell motions and their energetics. It also suggests that spatiotemporal synchronization might be one of the hallmarks of invasiveness of cancerous cells.

SUBMITTER: Hermans TM

PROVIDER: S-EPMC4122865 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Motility efficiency and spatiotemporal synchronization in non-metastatic vs. metastatic breast cancer cells.

Hermans Thomas M TM Pilans Didzis D Huda Sabil S Fuller Patrick P Kandere-Grzybowska Kristiana K Grzybowski Bartosz A BA

Integrative biology : quantitative biosciences from nano to macro 20131201 12

Metastatic breast cancer cells move not only more rapidly and persistently than their non-metastatic variants but in doing so use the mechanical work of the cytoskeleton more efficiently. The efficiency of the cell motions is defined for entire cells (rather than parts of the cell membrane) and is related to the work expended in forming membrane protrusions and retractions. This work, in turn, is estimated by integrating the protruded and retracted areas along the entire cell perimeter and is st ...[more]

PMID: 24136177

Similar Datasets

Project description:ObjectiveTo compare clinical outcomes of premature infants on synchronized nasal intermittent positive pressure ventilation (SNIPPV) vs nasal intermittent positive pressure ventilation (NIPPV) in the neonatal intensive care unit. Use of NIPPV in the neonatal intensive care unit has shown promise with better clinical outcomes in premature neonates. It is not known if synchronization makes a significant clinical impact when using this technique.Study designRetrospective data were obtained (1/04 to 12/09) of infants who received NIPPV anytime during their stay in the neonatal intensive care unit. SNIPPV (Infant Star with StarSync) was utilized from 2004 to 2006, whereas NIPPV (Bear Cub) was used from 2007 to 2009. Bronchopulmonary dysplasia (BPD) was defined using the NIH consensus definition. Unadjusted associations between potential risk factors and BPD/death were assessed using the χ (2) or Wilcoxon rank-sum test. Adjusted analyses were performed using generalized linear mixed models, taking into account correlation among infants of multiple gestation.ResultThere was no significant difference in the mean gestational age and birth weight in the two groups: SNIPPV (n=172; 27.0w; 1016 g) and NIPPV (n=238; 27.7w; 1117 g). There were no significant differences in maternal demographics, use of antenatal steroids, gender, multiple births, small for gestational age or Apgar scores in the two groups. More infants in the NIPPV group were given resuscitation in the delivery room (SNIPPV vs NIPPV: 44.2 vs 63%, P<0.001). Use of surfactant (84.4 vs 70.2%; P<0.001) was significantly higher in the SNIPPV group. There were no differences in the rate of patent ductus arteriosus, intraventricular hemorrhage, periventricular leukomalacia, retinopathy of prematurity and necrotizing enterocolitis in the two groups. After adjusting for the significant variables, use of NIPPV vs SNIPPV (odds ratio 0.74; 95% confidence interval: 0.42, 1.30) was not associated with BPD/death.ConclusionThese data suggest that use of SNIPPV vs NIPPV is not significantly associated with a differential impact on clinical outcomes.

Dataset Information

Motility efficiency and spatiotemporal synchronization in non-metastatic vs. metastatic breast cancer cells.

Publications

Motility efficiency and spatiotemporal synchronization in non-metastatic vs. metastatic breast cancer cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets