A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains.
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ABSTRACT:
SUBMITTER: Dokukin V
PROVIDER: S-EPMC4126072 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Publications
A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains.
Dokukin Victor V Silverman Scott K SK
Chemical communications (Cambridge, England) 20140801 66
We assess the utility of integrating a predetermined aptamer DNA module adjacent to a random catalytic DNA region for identifying new deoxyribozymes by in vitro selection. By placing a known ATP aptamer next to an N40 random region, an explicitly modular DNA catalyst for tyrosine side chain phosphorylation is identified. The results have implications for broader identification of deoxyribozymes that function with small-molecule substrates. ...[more]
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