Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation.
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ABSTRACT:
SUBMITTER: Levesque MJ
PROVIDER: S-EPMC4131260 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation.
Levesque Marshall J MJ Raj Arjun A
Nature methods 20130217 3
We report intron chromosomal expression FISH (iceFISH), a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We find substantial differences in transcriptional frequency between genes on a translocated chromosome and the same genes in their normal chromosomal context in the same cell. Correlations between genes on a single chromosome pointed toward a cis chromosome-level transcriptional interaction spanning 14.3 megabases. ...[more]
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