Dataset Information
Sequential determination of serum viral titers, virus-specific IgG antibodies, and TNF-?, IL-6, IL-10, and IFN-? levels in patients with Crimean-Congo hemorrhagic fever.
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ABSTRACT: Background
Although there have been a number of studies on the pathogenesis of Crimean-Congo hemorrhagic fever (CCHF) recently, knowledge on this topic is still insufficient. This study aims to reveal the kinetics of serum CCHF virus (CCHFV) titers, serum levels of anti-CCHFV immunoglobulin (Ig)G, tumor necrosis factor (TNF)-?, interleukin (IL)-6, IL-10, and interferon (IFN)-? in CCHF patients.Methods
In total, 31 CCHF cases (11 fatal) were studied. Serum samples were obtained daily from all patients from the time of admission and continued for a 7-day hospitalization period for serologic (ELISA), virologic (real-time PCR), and cytokine (ELISA) analysis.Results
The mean serum CCHFV titer at admission was 5.5E + 09 copies/mL in fatal cases and 5.7E + 08 copies/mL in survivors (p < 0.001). Compared to survivors, both the mean serum levels of IL-6 and TNF-? at admission were found to be significantly increased in fatal cases. The serum levels of IL-6, TNF-? and serum CCHFV titer at admission were significantly and positively correlated with disseminated intravascular coagulation (DIC) scores (r = 0.626, p = 0.0002; r = 0.461, p = 0.009; and r = 0.625, p = 0.003, respectively). When the data obtained from the sequential determination of CCHFV titer and levels of anti-CCHFV IgG, IL-6, TNF-?, IL-10 and IFN-? were grouped according to the days of illness, the initial serum CCHFV titer of a fatal patient was 5.5E + 09 (copies/mL) and it was 6.1E + 09 (copies/mL) in a survivor on the 2 day of illness. While significant alterations were observed in all cytokines during the monitoring period, IL-6 levels remained consistently higher in fatal cases and TNF-? levels increased in both in fatal and non-fatal CCHF cases.Conclusions
The increased CCHFV load and higher concentrations of IL-6 and TNF-?, the presence of DIC, and the absence of CCHFV specific immunity are strongly associated with death in CCHF.
SUBMITTER: Kaya S
PROVIDER: S-EPMC4133611 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Publications
Sequential determination of serum viral titers, virus-specific IgG antibodies, and TNF-α, IL-6, IL-10, and IFN-γ levels in patients with Crimean-Congo hemorrhagic fever.
BMC infectious diseases 20140728
<h4>Background</h4>Although there have been a number of studies on the pathogenesis of Crimean-Congo hemorrhagic fever (CCHF) recently, knowledge on this topic is still insufficient. This study aims to reveal the kinetics of serum CCHF virus (CCHFV) titers, serum levels of anti-CCHFV immunoglobulin (Ig)G, tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and interferon (IFN)-γ in CCHF patients.<h4>Methods</h4>In total, 31 CCHF cases (11 fatal) were studied. Serum samples were obtained da ...[more]