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High incidence and endemic spread of NDM-1-positive Enterobacteriaceae in Henan Province, China.


ABSTRACT: The emergence and spread of New Delhi metallo-?-lactamase 1 (NDM-1)-producing carbapenem-resistant Enterobacteriaceae (CRE) present an urgent threat to human health. In China, the bla(NDM-1 gene has been reported mostly in Acinetobacter spp. but is rarely found in Enterobacteriaceae. Here, we report a high incidence and endemic spread of NDM-1-producing CRE in Henan Province in China. Sixteen (33.3%) of the 48 CRE isolates obtained from patients during June 2011 to July 2012 were positive for bla(NDM-1), and the gene was found to be carried on plasmids of various sizes (? 55 to ? 360 kb). These plasmids were readily transferrable to recipient Escherichia coli by conjugation, conferred resistance to multiple antibiotics, and belonged to multiple replicon types. The bla(NDM-1)-positive CRE isolates were genetically diverse, and six new multilocus sequence typing (MLST) sequence types were linked to the carriage of NDM-1. Five of the isolates were classified as extensively drug-resistant (XDR) isolates, four of which also carried the fosA3 gene conferring resistance to fosfomycin, an alternative drug for treating infections by CRE. In each bla(NDM-1)-positive CRE isolate, the bla(NDM-1) gene was downstream of an intact ISAba125 element and upstream of the bleMBL gene. Furthermore, gene environment analysis suggested the possible transmission of bla(NDM-1)-containing sequences from Acinetobacter spp. to Klebsiella pneumoniae and Klebsiella oxytoca. These findings reveal the emergence and active transmission of NDM-1-positive CRE in China and underscore the need for heightened measures to control their further spread.

SUBMITTER: Qin S 

PROVIDER: S-EPMC4136005 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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High incidence and endemic spread of NDM-1-positive Enterobacteriaceae in Henan Province, China.

Qin Shangshang S   Fu Ying Y   Zhang Qijing Q   Qi Hui H   Wen Jian Guo JG   Xu Hui H   Xu Lijuan L   Zeng Li L   Tian Hao H   Rong Lijuan L   Li Yonghong Y   Shan Lihong L   Xu Hongde H   Yu Yunsong Y   Feng Xianju X   Liu Hong-Min HM  

Antimicrobial agents and chemotherapy 20140428 8


The emergence and spread of New Delhi metallo-β-lactamase 1 (NDM-1)-producing carbapenem-resistant Enterobacteriaceae (CRE) present an urgent threat to human health. In China, the bla(NDM-1 gene has been reported mostly in Acinetobacter spp. but is rarely found in Enterobacteriaceae. Here, we report a high incidence and endemic spread of NDM-1-producing CRE in Henan Province in China. Sixteen (33.3%) of the 48 CRE isolates obtained from patients during June 2011 to July 2012 were positive for bl  ...[more]

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