Development of an ex vivo porcine lung model for studying growth, virulence, and signaling of Pseudomonas aeruginosa.

ABSTRACT: Research into chronic infection by bacterial pathogens, such as Pseudomonas aeruginosa, uses various in vitro and live host models. While these have increased our understanding of pathogen growth, virulence, and evolution, each model has certain limitations. In vitro models cannot recapitulate the complex spatial structure of host organs, while experiments on live hosts are limited in terms of sample size and infection duration for ethical reasons; live mammal models also require specialized facilities which are costly to run. To address this, we have developed an ex vivo pig lung (EVPL) model for quantifying Pseudomonas aeruginosa growth, quorum sensing (QS), virulence factor production, and tissue damage in an environment that mimics a chronically infected cystic fibrosis (CF) lung. In a

SUBMITTER: Harrison F 

PROVIDER: S-EPMC4136229 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature