Dataset Information

Light-regulated gene repositioning in Arabidopsis.

ABSTRACT: Plant genomes are extremely sensitive to, and can be developmentally reprogrammed by environmental light cues. Here using rolling-circle amplification of gene-specific circularizable oligonucleotides coupled with fluorescence in situ hybridization, we demonstrate that light triggers a rapid repositioning of the Arabidopsis light-inducible chlorophyll a/b-binding proteins (CAB) locus from the nuclear interior to the nuclear periphery during its transcriptional activation. CAB repositioning is mediated by the red/far-red photoreceptors phytochromes (PHYs) and is inhibited by repressors of PHY signalling, including COP1, DET1 and PIFs. CAB repositioning appears to be a separate regulatory step occurring before its full transcriptional activation. Moreover, the light-inducible loci RBCS, PC and GUN5 undergo similar repositioning behaviour during their transcriptional activation. Our study supports a light-dependent gene regulatory mechanism in which PHYs activate light-inducible loci by relocating them to the nuclear periphery; it also provides evidence for the biological importance of gene positioning in the plant kingdom.

SUBMITTER: Feng CM

PROVIDER: S-EPMC4136543 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Light-regulated gene repositioning in Arabidopsis.

Feng Chun-Miao CM Qiu Yongjian Y Van Buskirk Elise K EK Yang Emily J EJ Chen Meng M

Nature communications 20140101

Plant genomes are extremely sensitive to, and can be developmentally reprogrammed by environmental light cues. Here using rolling-circle amplification of gene-specific circularizable oligonucleotides coupled with fluorescence in situ hybridization, we demonstrate that light triggers a rapid repositioning of the Arabidopsis light-inducible chlorophyll a/b-binding proteins (CAB) locus from the nuclear interior to the nuclear periphery during its transcriptional activation. CAB repositioning is med ...[more]

PMID: 24390011

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Dataset Information

Light-regulated gene repositioning in Arabidopsis.

Publications

Light-regulated gene repositioning in Arabidopsis.

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