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ABSTRACT:
SUBMITTER: Papaneophytou CP
PROVIDER: S-EPMC4137368 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Papaneophytou Christos P CP Grigoroudis Asterios I AI McInnes Campbell C Kontopidis George G
ACS medicinal chemistry letters 20140709 8
In order to quantify the interactions between molecules of biological interest, the determination of the dissociation constant (K d) is essential. Estimation of the binding affinity in this way is routinely performed in "favorable" conditions for macromolecules. Crucial data for ligand-protein binding elucidation is mainly derived from techniques (e.g., macromolecular crystallography) that require the addition of high concentration of salts and/or other additives. In this study we have evaluated ...[more]