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Generation of dipeptidyl peptidase-IV-inhibiting peptides from ?-lactoglobulin secreted by Lactococcus lactis.

ABSTRACT: Previous studies showed that hydrolysates of ?-lactoglobulin (BLG) prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV) activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system. Interestingly, trypsin-digested recombinant BLG from L. lactis inhibited DPP-IV activity, suggesting that BLG-secreting L. lactis may be useful in the treatment of type 2 diabetes mellitus.

SUBMITTER: Shigemori S

PROVIDER: S-EPMC4137494 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Generation of dipeptidyl peptidase-IV-inhibiting peptides from β-lactoglobulin secreted by Lactococcus lactis.

Shigemori Suguru S Oshiro Kazushi K Wang Pengfei P Yamamoto Yoshinari Y Wang Yeqin Y Sato Takashi T Uyeno Yutaka Y Shimosato Takeshi T

BioMed research international 20140803

Previous studies showed that hydrolysates of β-lactoglobulin (BLG) prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV) activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system. Interestingly, trypsin-digested recombinant BLG from L. lactis inhibited DPP-IV activity, suggesting that BLG-secreting L. lactis may be useful in the treatment of type 2 diabetes mellitus. ...[more]

PMID: 25157356

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