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Changes in nucleosome occupancy occur in a chromosome specific manner.

ABSTRACT: In the eukaryotic nucleus, DNA is packaged into chromatin. The fundamental subunit of chromatin is the nucleosome, DNA is wrapped 1.6 times around a histone octamer core. Nuclear processes in eukaryotes are impacted by whether regulatory DNA is occupied by nucleosomes. We used microarrays to measure nucleosome occupancy in human cells post Kaposi's sarcoma-associated herpesvirus (KSHV) reactivation at hundreds of immunity-related loci. The detailed analysis of these technologies can be found in recent publications from our lab (Druliner et al., 2013; Sexton et al., 2014). We found that nucleosome redistributions displayed chromosome specific nucleosome occupancy. This resource can be used to map nucleosome distributions in a variety of biological contexts.

SUBMITTER: Sexton BS

PROVIDER: S-EPMC4138544 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Changes in nucleosome occupancy occur in a chromosome specific manner.

Sexton Brittany S BS Druliner Brooke R BR Avey Denis D Zhu Fanxiu F Dennis Jonathan H JH

Genomics data 20141201

In the eukaryotic nucleus, DNA is packaged into chromatin. The fundamental subunit of chromatin is the nucleosome, DNA is wrapped 1.6 times around a histone octamer core. Nuclear processes in eukaryotes are impacted by whether regulatory DNA is occupied by nucleosomes. We used microarrays to measure nucleosome occupancy in human cells post Kaposi's sarcoma-associated herpesvirus (KSHV) reactivation at hundreds of immunity-related loci. The detailed analysis of these technologies can be found in ...[more]

PMID: 25152865

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