LEGO-NMR spectroscopy: a method to visualize individual subunits in large heteromeric complexes.
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ABSTRACT: Seeing the big picture: Asymmetric macromolecular complexes that are NMR active in only a subset of their subunits can be prepared, thus decreasing NMR spectral complexity. For the hetero heptameric LSm1-7 and LSm2-8 rings NMR spectra of the individual subunits of the complete complex are obtained, showing a conserved RNA binding site. This LEGO-NMR technique makes large asymmetric complexes accessible to detailed NMR spectroscopic studies.
SUBMITTER: Mund M
PROVIDER: S-EPMC4138990 | biostudies-literature |
REPOSITORIES: biostudies-literature
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