Dataset Information

A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.

ABSTRACT: A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% ?-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of the proinflammatory proteins tumor necrosis factor-? (TNF-?) and inducible nitric oxide synthase (iNOS) were increased in carrageenan-injected adult zebrafish during the development of abdominal edema. An associated enhancement was also observed in the leukocyte marker, myeloperoxidase (MPO). To support these results, we further observed that i.p. methylprednisolone (MP; 1 µg), a positive control, significantly inhibited carrageenan-induced inflammation 24 h after carrageenan administration. Furthermore, i.p. pretreatment with either an anti-TNF-? antibody (1?5 dilution in a volume of 20 µL) or the iNOS-selective inhibitor aminoguanidine (AG; 1 µg) inhibited carrageenan-induced abdominal edema in adult zebrafish. This new animal model is uncomplicated, easy to develop, and involves a straightforward inducement of inflammatory edema for the evaluation of small volumes of drugs or test compounds.

SUBMITTER: Huang SY

PROVIDER: S-EPMC4139260 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.

Huang Shi-Ying SY Feng Chien-Wei CW Hung Han-Chun HC Chakraborty Chiranjib C Chen Chun-Hong CH Chen Wu-Fu WF Jean Yen-Hsuan YH Wang Hui-Min David HM Sung Chun-Sung CS Sun Yu-Min YM Wu Chang-Yi CY Liu Wangta W Hsiao Chung-Der CD Wen Zhi-Hong ZH

PloS one 20140820 8

A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% λ-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of ...[more]

PMID: 25141004

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Dataset Information

A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.

Publications

A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.

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