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Bacterial and archaeal communities in Lake Nyos (Cameroon, Central Africa).

ABSTRACT: The aim of this study was to assess the microbial diversity associated with Lake Nyos, a lake with an unusual chemistry in Cameroon. Water samples were collected during the dry season on March 2013. Bacterial and archaeal communities were profiled using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) approach of the 16S rRNA gene. The results indicate a stratification of both communities along the water column. Altogether, the physico-chemical data and microbial sequences suggest a close correspondence of the potential microbial functions to the physico-chemical pattern of the lake. We also obtained evidence of a rich microbial diversity likely to include several novel microorganisms of environmental importance in the large unexplored microbial reservoir of Lake Nyos.

SUBMITTER: Tiodjio RE

PROVIDER: S-EPMC4139950 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Bacterial and archaeal communities in Lake Nyos (Cameroon, Central Africa).

Tiodjio Rosine E RE Sakatoku Akihiro A Nakamura Akihiro A Tanaka Daisuke D Fantong Wilson Y WY Tchakam Kamtchueng B KB Tanyileke Gregory G Ohba Takeshi T Hell Victor J VJ Kusakabe Minoru M Nakamura Shogo S Ueda Akira A

Scientific reports 20140821

The aim of this study was to assess the microbial diversity associated with Lake Nyos, a lake with an unusual chemistry in Cameroon. Water samples were collected during the dry season on March 2013. Bacterial and archaeal communities were profiled using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) approach of the 16S rRNA gene. The results indicate a stratification of both communities along the water column. Altogether, the physico-chemical data and microbial sequ ...[more]

PMID: 25141868

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