Ontology highlight
ABSTRACT:
SUBMITTER: Dalla Rosa I
PROVIDER: S-EPMC4140277 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Dalla Rosa Ilaria I Huang Shar-Yin N SY Agama Keli K Khiati Salim S Zhang Hongliang H Pommier Yves Y
The Journal of biological chemistry 20140505 26
Mitochondrial topoisomerase I (Top1mt) is a type IB topoisomerase present in vertebrates and exclusively targeted to mitochondria. Top1mt relaxes mitochondrial DNA (mtDNA) supercoiling by introducing transient cleavage complexes wherein the broken DNA strand swivels around the intact strand. Top1mt cleavage complexes (Top1mtcc) can be stabilized in vitro by camptothecin (CPT). However, CPT does not trap Top1mtcc efficiently in cells and is highly cytotoxic due to nuclear Top1 targeting. To map T ...[more]