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ABSTRACT: Background
Dickkopf-1 (DKK1) is an antagonist of Wnt/?-catenin signaling implicated in tumorigenesis. However, the biological role of DKK1 and ?-catenin involved in chondrosarcoma has not been sufficiently investigated. This study was designed to investigate the expression profiles of DKK1 and ?-catenin, and to clarify their clinical values in chondrosarcoma.Methods
The mRNA and protein levels of DKK1 and ?-catenin in fresh chondrosarcoma and the corresponding non-tumor tissues were analyzed by Real-time PCR and Western blot, respectively. The protein expression patterns of DKK1 and ?-catenin were investigated by immunohistochemistry. The associations among DKK1 level, ?-catenin accumulation, clinicopathological factors and the overall survival were separately evaluated.Results
Both DKK1 and ?-catenin levels were remarkably elevated in chondrosarcoma compared with the corresponding non-tumor tissues. High DKK1 level and positive ?-catenin accumulation in chondrosarcoma specimens were 58.7% and 53.9%, respectively. Elevated DKK1 level significantly correlated with positive ?-catenin accumulation, and they were remarkably associated with histological grade and Musculoskeletal Tumor Society stage. Furthermore, DKK1 level and ?-catenin accumulation had significant impacts on the prognosis of chondrosarcoma patients. Multivariate analysis revealed that DKK1 level was an independent prognostic factor for overall survival.Conclusions
Elevated DKK1 levels associated with ?-catenin accumulation play a crucial role in chondrosarcoma. DKK1 can serve as a novel predictor of poor prognosis in patients with chondrosarcoma.
SUBMITTER: Chen C
PROVIDER: S-EPMC4140757 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Chen Changbao C Zhou Hua H Zhang Xiaolin X Ma Xinlong X Liu Zhongjun Z Liu Xiaoguang X
PloS one 20140821 8
<h4>Background</h4>Dickkopf-1 (DKK1) is an antagonist of Wnt/β-catenin signaling implicated in tumorigenesis. However, the biological role of DKK1 and β-catenin involved in chondrosarcoma has not been sufficiently investigated. This study was designed to investigate the expression profiles of DKK1 and β-catenin, and to clarify their clinical values in chondrosarcoma.<h4>Methods</h4>The mRNA and protein levels of DKK1 and β-catenin in fresh chondrosarcoma and the corresponding non-tumor tissues w ...[more]