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Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes.

ABSTRACT: It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting factor Mer2 in the wake of the replication fork, synchronizing replication with an early prerequisite for DSB formation. Recruiting regulatory kinases to replisomes may be a general mechanism to ensure spatial and temporal coordination of replication with other chromosomal processes.

SUBMITTER: Murakami H

PROVIDER: S-EPMC4141489 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes.

Murakami Hajime H Keeney Scott S

Cell 20140801 4

It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting factor Mer2 in the wake of the replication fork ...[more]

PMID: 25126790

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