Ontology highlight
ABSTRACT:
SUBMITTER: McGlinchey RP
PROVIDER: S-EPMC4142984 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
McGlinchey Ryan P RP Jiang Zhiping Z Lee Jennifer C JC
Chembiochem : a European journal of chemical biology 20140620 11
Fibrils derived from Pmel17 are functional amyloids upon which melanin is deposited. Fibrils of the repeat domain (RPT) of Pmel17 form under strict melanosomal pH (4.5-5.5) and completely dissolve at pH≥6. To determine which Glu residue is responsible for this reversibility, aggregation of single, double, and quadruple Ala and Gln mutants were examined by intrinsic Trp fluorescence, circular dichroism spectroscopy, and transmission electron microscopy. Charge neutralization of E404, E422, E425, ...[more]