Ontology highlight
ABSTRACT:
SUBMITTER: Warner KD
PROVIDER: S-EPMC4143336 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Warner Katherine Deigan KD Chen Michael C MC Song Wenjiao W Strack Rita L RL Thorn Andrea A Jaffrey Samie R SR Ferré-D'Amaré Adrian R AR
Nature structural & molecular biology 20140715 8
GFP and its derivatives revolutionized the study of proteins. Spinach is a recently reported in vitro-evolved RNA mimic of GFP, which as genetically encoded fusions makes possible live-cell, real-time imaging of biological RNAs without resorting to large RNA-binding protein-GFP fusions. To elucidate the molecular basis of Spinach fluorescence, we solved the cocrystal structure of Spinach bound to its cognate exogenous chromophore, showing that Spinach activates the small molecule by immobilizing ...[more]