Ontology highlight
ABSTRACT:
SUBMITTER: Gori SS
PROVIDER: S-EPMC4144182 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Gori Sadakatali S SS Lorkiewicz Pawel P Ehringer Daniel S DS Belshoff Alex C AC Higashi Richard M RM Fan Teresa W-M TW Nantz Michael H MH
Analytical and bioanalytical chemistry 20140525 18
We describe preparation and use of the quaternary ammonium-based α-iodoacetamide QDE and its isotopologue *QDE as reagents for chemoselective derivatization of cellular thiols. Direct addition of the reagents to live cells followed by adduct extraction into n-butanol and analysis by FT-ICR-MS provided a registry of matched isotope peaks from which molecular formulae of thiol metabolites were derived. Acidification to pH 4 during cell lysis and adduct formation further improves the chemoselectivi ...[more]