Hotspot-centric de novo design of protein binders.
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ABSTRACT:
SUBMITTER: Fleishman SJ
PROVIDER: S-EPMC4144197 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
Hotspot-centric de novo design of protein binders.
Fleishman Sarel J SJ Corn Jacob E JE Strauch Eva-Maria EM Whitehead Timothy A TA Karanicolas John J Baker David D
Journal of molecular biology 20110910 5
Protein-protein interactions play critical roles in biology, and computational design of interactions could be useful in a range of applications. We describe in detail a general approach to de novo design of protein interactions based on computed, energetically optimized interaction hotspots, which was recently used to produce high-affinity binders of influenza hemagglutinin. We present several alternative approaches to identify and build the key hotspot interactions within both core secondary s ...[more]
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