A robust system for production of minicircle DNA vectors.
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ABSTRACT:
SUBMITTER: Kay MA
PROVIDER: S-EPMC4144359 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Publications
A robust system for production of minicircle DNA vectors.
Kay Mark A MA He Cheng-Yi CY Chen Zhi-Ying ZY
Nature biotechnology 20101121 12
Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of p ...[more]
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